ACD Seminar Series
University of Cambridge, UK - CAB
July 20, 2017
Room: Lecture Hall at CAB
10:00 - 11:30h

COMPLETE TO REGISTER

 


Single-molecule RNA in situ hybridization for the detection of gene expression and alternative splicing in tissue

 

Thursday, July 20, 2017

10:00 to 11:30 
 

University of Cambridge
Clifford Albutt Building (CAB)
Cambridge Biomedical Campus
Hills Road, Cambridge, CB2 0AH, UK

 

Room: Lecture Hall


 

Single-molecule RNA in situ hybridization for the detection of gene expression and alternative splicing in tissue

 

RNA in situ hybridization is a powerful technique for analyzing RNA expression at the single cell level within the morphological context, providing valuable spatial and cellular information missing from bulk tissue analysis.

The next-generation RNA ISH technologies called RNAscope® and BaseScope™ allow single RNA molecule detection in single cells and tissues with single-base resolution. 

Key features
 

  • Detection of a single RNA molecules. The RNAscope® probe design provides robustness against partial target RNA accessibility or degradation.
  • Signal amplification coupled with simultaneous background suppression strategy results in single-RNA-molecule detection even in partially degraded samples.
  • Provides cell-specific expression information in intact tissue architecture.
  • Works for virtually ANY gene from ANY species in ANY tissue.

Examples of applications of RNAscope® and BaseScope™ assay will be discussed, including

  • GPCRs and secreted molecules
  • Alternative splicing
  • Circular RNA

 

Speaker

Dr. Emily Park
Senior Director of Scientific Affairs at Advanced Cell Diagnostics