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Single-molecule RNA in situ hybridization for the detection of gene expression and alternative splicing in tissue
Thursday, July 20, 2017
12:00 to 14:00
University of Cambridge
Li Ka Shing Centre
RNA in situ hybridization is a powerful technique for analyzing RNA expression at the single cell level within the morphological context, providing valuable spatial and cellular information missing from bulk tissue analysis.
The next-generation RNA ISH technologies called RNAscope® and BaseScope™ allow single RNA molecule detection in routine clinical formalin-fixed paraffin-embedded (FFPE) tissue with single-base resolution.
- Detection of a single RNA molecules. The RNAscope® probe design provides robustness against partial target RNA accessibility or degradation.
- Signal amplification coupled with simultaneous background suppression strategy results in single-RNA-molecule detection even in partially degraded samples.
- Provides cell-specific expression information in intact tissue architecture.
- Works for virtually ANY gene from ANY species in ANY tissue.
Examples of applications of RNAscope® and BaseScope™ assay will be discussed, including
- Characterization of tumor, stromal, and immune cell states in tumor microenvironment
- Revealing tumor intrinsic expression of immune checkpoint molecules
- Characterization of alternative splicing
Dr. Emily Park
Senior Director of Scientific Affairs at Advanced Cell Diagnostics