ACD Seminar Series
University of Cambridge, UK
July 20, 2017
Room: 009
12:00 - 14:00h

COMPLETE TO REGISTER


Single-molecule RNA in situ hybridization for the detection of gene expression and alternative splicing in tissue 

 

Thursday, July 20, 2017

12:00 to 14:00 
 

University of Cambridge
Li Ka Shing Centre
Robinson Way
Cambridge

Room: 009


 

RNA in situ hybridization is a powerful technique for analyzing RNA expression at the single cell level within the morphological context, providing valuable spatial and cellular information missing from bulk tissue analysis.

The next-generation RNA ISH technologies called RNAscope® and BaseScope™ allow single RNA molecule detection in routine clinical formalin-fixed paraffin-embedded (FFPE) tissue with single-base resolution. 

Key features
 

  • Detection of a single RNA molecules. The RNAscope® probe design provides robustness against partial target RNA accessibility or degradation.
  • Signal amplification coupled with simultaneous background suppression strategy results in single-RNA-molecule detection even in partially degraded samples.
  • Provides cell-specific expression information in intact tissue architecture.
  • Works for virtually ANY gene from ANY species in ANY tissue.

Examples of applications of RNAscope® and BaseScope™ assay will be discussed, including

  • Characterization of tumor, stromal, and immune cell states in tumor microenvironment
  • Revealing tumor intrinsic expression of immune checkpoint molecules
  • Characterization of alternative splicing

 

Speaker

Dr. Emily Park
Senior Director of Scientific Affairs at Advanced Cell Diagnostics