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Thursday June 1, 2017
13:30 - 14:30 pm
Seminar Room 4, Ground Floor, Francis Crick Building
The Francis Crick Insitute, London, UK
Visualize Gene Expression & Genetic Variations in Tissues:
Applications of RNAscope® and BaseScopeTM ISH technology
RNAscope® is a proprietary RNA in situ hybridization (ISH) assay based on ACD patented signal amplification and background suppression technology which advances RNA biomarker analysis in tissues and cells. Unique to this technology, RNAscope® delivers quantitative, sensitive and specific molecular detection of RNA species on a cell-by-cell basis with morphological context in a single assay. This enables researchers to visualize which genes are expressed, localize where they are expressed, and quantify the level of expression.
- Detection of a single RNA molecule requires only three double Z probe pairs to bind to the target RNA molecule. The RNAscope® 20 double Z probe pairs design provides robustness against partial target RNA accessibility or degradation.
- Signal amplification coupled with simultaneous background suppression strategy results in single-RNA-molecule detection even in partially degraded samples. Rigorous double Z probes design eliminates cross hybridization to unintended targets and routinely distinguishes RNA sequences with up to 85% homology.
- Provides cell-specific expression information in intact tissue architecture.
- Works for virtually ANY gene from ANY species in ANY tissue.
Faraia Shah (MPhil, PhD)
FShah@acdbio.com, Sales Account Executive, Advanced Cell Diagnostics
Advances in research : Visualising gene expression and genetic variations in the tissue environment
Ann-Marie Baker PhD
Postdoctoral Research Assistant, Bart Cancer Institute
'Investigating cancer evolution and heterogeneity using in situ hybridisation’
Space is limited. Cakes and buns will be provided so please register for headcount.