ACD Seminar Series
RNAscope® Technology

June 9th, 2017

MRC Weatherall Institute of Molecular Medicine (WIMM)
Oxford University

 

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RNASCOPE® TECHNOLOGY 

June 9, 2017

12 - 1 pm

MRC Weatherall Institute of Molecular Medicine (WIMM)
University of Oxford
John Radcliffe Hospital
Oxford, UK


 

The RNAscope® technology is being routinely used for tissue-based gene expression analysis for target validation, pathobiology and biomarker development in every disease area. This technology, with its capacity to provide single-cell and single-RNA molecule sensitivity in the context of complex tissue microenvironments, is an ideal tool to complement technologies like IHC, qPCR or NGS.

 

Matthew Nolan will present on the RNAscope® application in Neuroscience “In-situ expression of post-mitotic transcription factors associated with projection neuron diversity in the adult post-mortem brain

 

In addition Faraia Shah will give an overview on:

Visualizing gene expression and genetic variations in tissue environments of Neuroscience and Cancer related research by RNAscope® and BaseScope™

  • Detect mRNAs and lncRNAs at single molecule sensitivity by RNAscope® assay
  • Target specific splice junctions for aberrant alternative splicing in cancers by BaseScope™ assay
  • Characterize expression profiles of various checkpoint markers and therapeutic targets in the tumor microenvironment (TME)
  • Detection, characterization, and (co-)localization of mRNAs in the central and peripheral nervous system:
    • Co-expression with specific cell type markers
    • Sub-cellular localization of mRNA expression in cell body, axons or dendrites 
  • Distinguish specific homologues, paralogues, and orthologues by RNAscope® assay or BaseScope™ assay
  • Perform single cell analysis with morphological information in FFPE or frozen tissue
  • Pinpoint expression of novel RNAs of interest 

Speaker

Matthew Nolan (MSc)
D.Phil candidate in clinical neuroscience 
at the University of Oxford

 

Faraia Shah (MPhil, PhD)
Sales Account Executive
Advanced Cell Diagnostics